Cholesterol Esterase is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
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Synthesis of fatty acid esters of cholesterol
|Alternate Names||Bile salt activated lipase; sterol esterase; carboxyl ester lipase; steryl-ester acylhydrolase; Cholesterase; Neutral cholesteryl ester hydrolase|
|Application Notes||Cholesterol Esterase is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.|
|Base Catalog Number||105439|
|Biochemical Physiological Actions||Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The enzyme may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis.|
|Isoelectric Point||5.9 ± 0.1(Lit.)|
|Personal Protective Equipment||Eyeshields, Gloves, Respirator filter.|
|Protein or Enzyme Type||Proteins, Enzymes & Peptides|
|Specific Activity||≥100 u/mg solid|
|Unit Definition||One unit is the amount of enzyme that will hydrolyze 1.0 micromole of cholesterol ester per minute at 37 °C, pH 7.0.|