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PBR322 plasmid , 25 µg

PBR322 plasmid , 25 µg

CN¥0.00

SKU: 04821225

MDL Number:
MFCD00146650
Packaging size:
25 mu;g
Product Description

pBR322 is a 4363 bp vector purified from Escherichia coli.It is one of the most commonly used cloning vectors, that confers resistance to ampicillin and tetracycline. It contains single restriction sites for Pst I and Pvu I within the ampicillin-resistance gene (ampr) and single restriction sites for BamH I, Sal I, Sph I, EcoR V, Nru I and Xma III within the tetracycline-resistance gene (tetr).

Application Notes

Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication. Note-Elevated temperatures and/or multiple freeze/thaw cycles will cause some of the ccDNA to convert to ocDNA.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Cloning vector

Specifications
SKU 04821225
Application Notes Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication. Note-Elevated temperatures and/or multiple freeze/thaw cycles will cause some of the ccDNA to convert to ocDNA.
Base Catalog Number 821225
Pack Size 25 mu;g
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Solubility Soluble in Water.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)