A redox indicator and reagent for spectrophotometric assay of silver. It forms a complex with ferrous (iron II, Fe2+) ion; it can be used as an indicator in oxidation-reduction systems, in titrating ferrous salts. The product forms a complex with Fe2+, but not with Fe3+. NH2OH can be added to the solution to reduce the Fe3+ to Fe2+ for analysis of iron. The complex between iron (II) and 1,10-phenanthroline can be quantitated by its absorption at 510 nm. Also chelates other dilvalent metals. Due to the compound's strong UV absorbance, it may interfere with spectrophotometric assays. When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions.
When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions.
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|Alternate Names||o-Phenanthroline; 4,5-Diazaphenanthrene; 1,10-Fenanthrolin; 1,10-Fenantrolina; beta-Phenanthroline; 1,10-Phenanthrolineo-phenanthroline|
|Application Notes||When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions.|
|Base Catalog Number||15255305|
|Beilstein Registry Number||4008096|
|Biochemical Physiological Actions||Metalloprotease inhibitor; chelates iron, zinc and other divalent metals.|
|Extinction Coefficient||31.5 (solvent not stated)|
|Melting Point||100-104°C (lit.)|
|Molecular Formula||C12H8N2 • H2O|
|Pack Size||5 g|
|Personal Protective Equipment||dust mask, Eyeshields, Faceshields, Gloves, respirator cartridges|
|Safety Symbol||GHS06, GHS09|
|Solubility||Soluble in methanol, ethanol or acetone. Also soluble in 300 parts water or 70 parts benzene.|
|Typical Working Concentration||A metallo-protease inhibitor; effective concentration is 1-10 mM.|