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Ribonuclease, beef pancreas, 100 mg

Ribonuclease, beef pancreas, 100 mg

CN¥0.00

SKU: 02101075.1

Synonyms
RNase A; E.C. 2.7.7.16; Ribonuclease I; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase; Pancreatic Ribonuclease; Endoribonuclease I
CAS Number:
9001-99-4
Molecular Weight:
~13,700 (Lit.)
EC Number:
232-646-6
MDL Number:
MFCD00132181
Packaging size:
100 mg
Product Description

RNase A is an endoribonuclease that attacks at the 3' phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges.

Application Notes

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the tissue-specific expression of ruminant pancreatic type ribonucleases. Ribonuclease A from bovine pancreas has also been used in a study to investigate pancreas-specific glycosylated protein disulphide-isomerase binding to misfolded proteins and peptides.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Purification of DNA | RNA sequence analysis | RNase protection assays | Remove unspecifically bound RNA | Hydrolyze RNA contained in protein samples

Specifications
SKU 02101075.1
Alternate Names RNase A; E.C. 2.7.7.16; Ribonuclease I; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase; Pancreatic Ribonuclease; Endoribonuclease I
Application Notes Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the tissue-specific expression of ruminant pancreatic type ribonucleases. Ribonuclease A from bovine pancreas has also been used in a study to investigate pancreas-specific glycosylated protein disulphide-isomerase binding to misfolded proteins and peptides.
Base Catalog Number 101075.1
Biochemical Physiological Actions Ribonuclease A cleaves single stranded RNA after pyrimidine nucleotides.
CAS # 9001-99-4
EC Number 232-646-6
Extinction Coefficient E1%280 = 7.3(Lit.)
Hazard Statements H317
Molecular Weight ~13,700 (Lit.)
Pack Size 100 mg
Personal Protective Equipment Eyeshields, Gloves, respirator filter
pH Optimum pH: 7.0 - 7.5(Lit.)
RTECS Number RF0760000
Solubility Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution.
Specific Activity ~70 Kunitz u/mg solid
Unit Definition One unit causes the hydrolysis of RNA at a rate such that the velocity constant (k) equals 1 at 25 °C and pH 5.0.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
Documents

Datasheet

Datasheet