G418 Sulfate is an aminoglycoside related to Gentamicin and is toxic to bacteria, yeast,higher plants, mammalian cells, protozoans and helminths. The resistance genes are located on transposons Tn601(903) and Tn5 and are bacterial in derivation, but they can be expressed in eukaryotic cells. By introducing these genes into cells, resistance to G418 Sulfate is conferred. G418 Sulfate is an effective selecting agent utilized in killing prokaryotic and eukaryotic cells.
G418 Sulfate is used as a selective antibiotic in the concentration range of 100 - 200 µg/mL for bacteria, or 200 – 500 µg/mL for most mammalian cells. It is also used for the selection of transfected mammalian cells, yeast, dictyostelium, plant and bacteria.
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Cell culture applications | Antimicrobial Treatment | Eukaryotic Selection | Stable Cell Line Generation
|Alternate Names||Antibiotic G418; Geneticin®|
|Application Notes||G418 Sulfate is used as a selective antibiotic in the concentration range of 100 - 200 µg/mL for bacteria, or 200 – 500 µg/mL for most mammalian cells. It is also used for the selection of transfected mammalian cells, yeast, dictyostelium, plant and bacteria.|
|Base Catalog Number||1672546,1672548|
|Concentration||Concentration (active G418 sulfate) 50 ± 1 mg/mL|
|Solubility||Soluble in water|
|Sterility||Sterile | Filtered through .22 µm micron filter|
|Typical Working Concentration||100 - 200 µg ⁄ mL bacteria, 200 – 500 µg ⁄ mL mammalian cells|