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Tris, molecular biology reagent, ≥99.95%

Tris, molecular biology reagent, ≥99.95%

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Key features and details

Tris

SKU: 02194855-CF

Synonyms
Trometamol; Tromethamine; Tris-[hydroxymethyl]amino- methane; THAM; 2-Amino-2-(hydroxymethyl)- 1,3-propandiol; THAM; Tris base
CAS Number:
77-86-1
Molecular Formula:
C4H11NO3
Molecular Weight:
121.136 g/mol
Beilstein Registry Number:
741883
EC Number:
201-064-4
MDL Number:
MFCD00004679
Product Description

Tris

Application Notes

Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). Tris does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is an auxiliary material in pharmaceutical science.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Buffer | Used as a starting material for polymers; oxazolones (with carboxylic acids) and oxazolidines | Used in direct standardization of a strong acid solution

Specifications
SKU 02194855-CF
Alternate Names Trometamol; Tromethamine; Tris-[hydroxymethyl]amino- methane; THAM; 2-Amino-2-(hydroxymethyl)- 1,3-propandiol; THAM; Tris base
Application Notes Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). Tris does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is an auxiliary material in pharmaceutical science.
Base Catalog Number 194855
Beilstein Registry Number 741883
Boiling Point 219-220 deg C at 10 mm Hg
CAS # 77-86-1
EC Number 201-064-4
Format Crystalline powder
Grade Molecular Biology Grade
Hazard Statements H315-H319-H335
Melting Point 171-172 deg C
Molecular Formula C4H11NO3
Molecular Weight 121.136 g/mol
Personal Protective Equipment Dust mask, Eyeshields, Gloves
pH pH of 0.1 molar aq soln = 10.4
pKa 8.1 at 25 °C(Lit.)
Purity ≥95%
RTECS Number TY2900000
Safety Symbol GHS07
Solubility 79.1 mg/mL in <a class="pubchem-internal-link CID-174" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethylene%20glycol">ethylene glycol</a>; 26 mg/mL in <a class="pubchem-internal-link CID-887" href="https://pubchem.ncbi.nlm.nih.gov/compound/methanol">methanol</a>; 14.6 mg/mL in anhyd <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>; 22 mg/mL in 95% <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>; 14 mg/mL in <a class="pubchem-internal-link CID-6228" href="https://pubchem.ncbi.nlm.nih.gov/compound/dimethyl-formamide">dimethyl formamide</a>; 20 mg/mL in <a class="pubchem-internal-link CID-180" href="https://pubchem.ncbi.nlm.nih.gov/compound/acetone">acetone</a>; 0.5 mg/mL in <a class="pubchem-internal-link CID-8857" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethyl%20acetate">ethyl acetate</a>; 0.4 mg/mL in olive oil; 0.1 mg/mL in <a class="pubchem-internal-link CID-8078" href="https://pubchem.ncbi.nlm.nih.gov/compound/cyclohexane">cyclohexane</a>; 0.05 mg/mL in <a class="pubchem-internal-link CID-6212" href="https://pubchem.ncbi.nlm.nih.gov/compound/chloroform">chloroform</a>; less than 0.05 mg/mL in <a class="pubchem-internal-link CID-5943" href="https://pubchem.ncbi.nlm.nih.gov/compound/carbon%20tetrachloride">carbon tetrachloride</a>
Sterilization of Solutions Tris solutions can be autoclaved (121 °C, 15 psi, 15 minutes) or sterile filtered.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance OD 260 nm (5% aq soln) ≤0.1
Vapor Pressure 2.2X10-5 mm Hg at 25 deg C (est)
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