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Alkaline phosphatase

Alkaline phosphatase

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Key features and details

Alkaline Phosphatase

SKU: 02190680-CF

Synonyms
EC 3.1.3.1; Alkaline phosphomonoesterase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); CIAP
CAS Number:
9001-78-9
Molecular Weight:
496.569 g/mol
EC Number:
232-631-4
MDL Number:
MFCD00131849
Product Description

Alkaline Phosphatase

Application Notes

Alkaline phosphatase is used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations. It is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations | Used in immunoblotting; ELISA′s; and other antibody based detection assays

Specifications
SKU 02190680-CF
Alternate Names EC 3.1.3.1; Alkaline phosphomonoesterase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); CIAP
Application Notes Alkaline phosphatase is used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations. It is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.
Base Catalog Number 190680
Biochemical Physiological Actions Catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.
CAS # 9001-78-9
EC Number 232-631-4
Format Powder
Isoelectric Point 5.7(Lit.)
Molecular Weight 496.569 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Source Calf Intestine
Specific Activity ≥1.0 u/mg
Unit Definition That amount of enzyme causing the hydrolysis of one micromole of p-nitrophenyl phosphate per minute at 25 °C and pH 9.6.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

Certificates of Analysis

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