Alkaline phosphatase is used for removing phosphate monoester to prevent self ligation. It can be also be used as a label for enzyme immunoassays.
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|Alternate Names||Orthophosphoric Monoester Phosphohydrolase; Alkaline phosphomonoesterase|
|Application Notes||Alkaline phosphatase is used for removing phosphate monoester to prevent self ligation. It can be also be used as a label for enzyme immunoassays.|
|Base Catalog Number||159347|
|Molecular Weight||496.569 g/mol|
|Unit Definition||One unit liberates one µmole of p-nitrophenol per minute at 37 °C, pH 10.15 in Tris buffer. The enzyme activity is determined by measuring the increase in optical density at 405 nm resulting from the hydrolysis of p-nitrophenyl phosphate.|