Cart
 
In Stock

Carbonic anhydrase

Carbonic anhydrase

CN¥0.00

Key features and details

Carbonic Anhydrase

SKU: 02153879-CF

Synonyms
Carbonate Dehydratase; Carbonate Hydrolyase
CAS Number:
9001-03-0
Molecular Weight:
397.751 g/mol
EC Number:
232-576-6
MDL Number:
MFCD00081467
Product Description

Carbonic Anhydrase

Application Notes

Carbonic anhydrase from bovine erythrocytes (BCA) has been used to study the effect of removal of enzyme-bound metal ion, Zn2+, on aggregation behavior of the enzyme. Removal of metal ion by a chelator such as EDTA enhances the propensity of the enzyme to adopt the molten-globule state. This state of the enzyme is found to bind to the chaperone-like α-crystallin and prevent aggregation. The enzyme from Sigma has been immobilized to electrochemical transducers in order to obtain develop an analytical device for dissolved CO2 measurement. It has also been used for the thermodynamic analysis of conformational changes in BCA.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Enzymes | Inhibitors | Substrates

Specifications
SKU 02153879-CF
Alternate Names Carbonate Dehydratase; Carbonate Hydrolyase
Application Notes Carbonic anhydrase from bovine erythrocytes (BCA) has been used to study the effect of removal of enzyme-bound metal ion, Zn2+, on aggregation behavior of the enzyme. Removal of metal ion by a chelator such as EDTA enhances the propensity of the enzyme to adopt the molten-globule state. This state of the enzyme is found to bind to the chaperone-like α-crystallin and prevent aggregation. The enzyme from Sigma has been immobilized to electrochemical transducers in order to obtain develop an analytical device for dissolved CO2 measurement. It has also been used for the thermodynamic analysis of conformational changes in BCA.
Base Catalog Number 153879
Biochemical Physiological Actions Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications for various pathologies such as glaucoma, epilepsy and Parkinson’s disease.
CAS # 9001-03-0
EC Number 232-576-6
Format Powder
Hazard Statements H317
Molecular Weight 397.751 g/mol
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Source Bovine Erythrocytes
Specific Activity >2000 u/mg solid
Unit Definition The Unit is determined by the electrometic method in which the time required (in seconds) for a saturated CO2 solution to lower the pH of 0.02M Tris-HCI buffer from 8.3 to 6.3 at 0 °C is determined. The time without enzyme is recorded at To; with enzyme T. One unit equals 2X (To-T)/T using the electrometric assay.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)

Material Safety Data Sheet

Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United Kingdom)
Material Safety Data Sheet
Japanese (Japan)
Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (Australia)