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Riboflavin

Riboflavin

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Key features and details

Riboflavin

SKU: 02102813-CF

Synonyms
Vitamin B2; Lactoflavin; Vitamin G; 7,8-Dimethyl-10- (D-ribo-2,3,4,5- tetrahydroxypentyl) isoalloxazine; 7,8-Dimethyl-10-ribitylisoalloxazine
CAS Number:
83-88-5
Molecular Formula:
C17H2ON4O6
Molecular Weight:
376.369 g/mol
Beilstein Registry Number:
97825
EC Number:
201-507-1
Product Description

Riboflavin

Application Notes

Used as a photoinitiator for polymerization of polyacrylamide gels. It forms free radicals in aqueous solution in the presence of light. Riboflavin photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen but traces of oxygen allows for leucoflavin to reoxidize with free-radical generation. The catalysts TEMED or DMAPN are commonly added to speed up the free radical formation. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for electrophoresis. Riboflavin is commonly used in the stacking gel for non-denaturing polyacrylamide electrophoresis because native proteins can be senstive to persulfate ions from ammonium persulfate. Another advantage of riboflavin over ammonium persulfate is that it will not start polymerization until the gel is illuminated.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Specifications
SKU 02102813-CF
Alternate Names Vitamin B2; Lactoflavin; Vitamin G; 7,8-Dimethyl-10- (D-ribo-2,3,4,5- tetrahydroxypentyl) isoalloxazine; 7,8-Dimethyl-10-ribitylisoalloxazine
Application Notes Used as a photoinitiator for polymerization of polyacrylamide gels. It forms free radicals in aqueous solution in the presence of light. Riboflavin photodecomposes to leucoflavin. No free radicals are formed in the absence of oxygen but traces of oxygen allows for leucoflavin to reoxidize with free-radical generation. The catalysts TEMED or DMAPN are commonly added to speed up the free radical formation. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for electrophoresis. Riboflavin is commonly used in the stacking gel for non-denaturing polyacrylamide electrophoresis because native proteins can be senstive to persulfate ions from ammonium persulfate. Another advantage of riboflavin over ammonium persulfate is that it will not start polymerization until the gel is illuminated.
Base Catalog Number 102813
Beilstein Registry Number 97825
CAS # 83-88-5
EC Number 201-507-1
Format Powder
Grade USP
Melting Point 280 dec °C
Molecular Formula C17H2ON4O6
Molecular Weight 376.369 g/mol
Optical Rotation (c = 0.5, 0.05 M NaOH)
Particle Size -115° to -135°
pH pH of saturated aqueous solution: about 6
RTECS Number VJ1400000
Safety Symbol ≤0.3%
Solubility <h5 class="minor-section-title">Water Solubility</h5>84.7 mg/L (at 25 °C)
Source Soluble in 0.1 M Sodium Hydroxide (50 mg/ml - clear; yellowish-orange solution); only slightly soluble in water.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
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Datasheet

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