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P-Nitro blue tetrazolium chloride

P-Nitro blue tetrazolium chloride

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Key features and details

P-Nitro Blue Tetrazolium Chloride

SKU: 02100416-CF

Synonyms
2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride; 3,3'-(3,3'-Dimethoxy-4,4'-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; NBT; Nitro BT
CAS Number:
298-83-9
Molecular Formula:
C40H30CI2N10O6
Molecular Weight:
817.644 g/mol
Beilstein Registry Number:
4115923
EC Number:
206-067-4
MDL Number:
MFCD00012159
Product Description

P-Nitro Blue Tetrazolium Chloride

Application Notes

p - nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Colorimetric detection of alkaline phosphatase conjugates

Specifications
SKU 02100416-CF
Alternate Names 2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride; 3,3'-(3,3'-Dimethoxy-4,4'-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; NBT; Nitro BT
Application Notes p - nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.
Base Catalog Number 100416
Beilstein Registry Number 4115923
CAS # 298-83-9
EC Number 206-067-4
Extinction Coefficient 260 nm: E1% = 740 (in water) (Lit.)
Format Powder
Hazard Statements H302-H370
Molecular Formula C40H30CI2N10O6
Molecular Weight 817.644 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
RTECS Number XF8045000
Safety Symbol GHS07, GHS08
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance 255 ± 3 nm ( λ max (MeOH))
Citations
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