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IgG2A (Kappa) UPC 10, mouse myeloma, purified, 1 mg

IgG2A (Kappa) UPC 10, mouse myeloma, purified, 1 mg

CN¥0.00

Key features and details

Mouse IgG2a (Kappa) purified from clarified mouse UPC 10 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02 M Tris, 0.15 M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

SKU: 0850328

Synonyms
Mouse IgG2a/k
MDL Number:
MFCD00164800
Packaging size:
1 mg
Product Description

Product is purified mouse IgG2a (Kappa) and buffer. Mouse IgG2a (Kappa) is purified from clarified mouse UPC 10 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

Application Notes

This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | ELISA | Control

Specifications
SKU 0850328
Alternate Names Mouse IgG2a/k
Antibody Type Monoclonal Antibody
Application Notes This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
Base Catalog Number 50328
Clone Name UPC 10
Concentration Total Protein: 5.0 mg/mL
Conjugate Unconjugated
Formulation Details Purified
Host Mouse
Isotype IgG
Pack Size 1 mg
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Preparation Method Mouse IgG2a (j) is purified from clarified mouse UPC 10 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.01M Sodium Phosphate, 0.13M sodium chloride, pH 7.2, and vialed.
Purity Affinity Purified
Solubility Soluble in Water
Specificity The total protein is determined by absorbance at 280nm. The product contains 1 mg of mouse IgG2a. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgG2a; no trace of IgG1; IgG2b; IgG3; IgA; IgM or non-immunoglobulin serum proteins is detected.
Sterility Filtered through a .22 µm filter
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)

Material Safety Data Sheet

Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United Kingdom)
Material Safety Data Sheet
English (United States)