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Anti-human IgG F(Ab')2 goat IgG fraction, peroxidase-conjugated , 2 mL

Anti-human IgG F(Ab')2 goat IgG fraction, peroxidase-conjugated , 2 mL

CN¥0.00

Key features and details

Lyophilized horseradish peroxidase (HRP)-conjugated goat IgG fraction to human IgG F(ab')2 and buffer salts.

SKU: 0855224

Packaging size:
2 mL
Product Description

Peroxidase-Conjugated Goat IgG Fraction to Human IgG F(ab')2 is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to human IgG F(ab')2 and buffer salts.

Application Notes

Peroxidase-Conjugated Goat IgG Fraction to Human IgG F(ab')2 is suitable for use as a reagent in enzyme immunoassays (EIA), cell and immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. Also used in Immunohistochemistry, EIA and Western blots.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications
SKU 0855224
Antibody Type Polyclonal Antibody
Application Notes Peroxidase-Conjugated Goat IgG Fraction to Human IgG F(ab')2 is suitable for use as a reagent in enzyme immunoassays (EIA), cell and immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. Also used in Immunohistochemistry, EIA and Western blots.
Base Catalog Number 55224
Concentration Total Protein: 5.9 mg/mL; Titer: 1:192;000
Conjugate HRP
Formulation Details Lyophilized
Host Goat
Immunogen Purified human IgG
Pack Size 2 mL
Preparation Method Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Purity Purified
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human, IgG F(Ab')2
Specificity It shows reactivity to human IgG F(ab')<sub>2</sub>; cross-reactivity to other species may exist. Because the product is directed against F(ab')<sub>2</sub>; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Typical Working Concentration Immunohistochemistry: 1:500 - 1:5000; EIA and Western blots: 1:5000 - 1:100,000. Dilution recommended: 1:500 - 1:4,000 for Tissue Staining; 1:1,000 - 1:8,000 for Immuno Blotting; 1:2,000 - 1:16,000 for ELISA/FIA
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
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