Cart
 
Inquire

Anti-mouse complement C3 goat IgG fraction, fluorescein-conjugated, 2 mL

Anti-mouse complement C3 goat IgG fraction, fluorescein-conjugated, 2 mL

CN¥0.00

Key features and details

Lyophilized fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to mouse complement C3 and buffer salts.

SKU: 0855500

Packaging size:
2 mL
Product Description

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to mouse complement C3 and buffer salts.

Application Notes

Fluorescein-Conjugated Goat IgG to Mouse Complement C3 is intended for laboratory use to qualitatively stain sections of frozen tissue, suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunofluorescence assays (IFA) | Immunoblot

Specifications
SKU 0855500
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-Conjugated Goat IgG to Mouse Complement C3 is intended for laboratory use to qualitatively stain sections of frozen tissue, suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Base Catalog Number 55500
Concentration Total Protein: 8.3 mg/mL; Antibody Protein: 4 mg/mL
Conjugate FITC
Formulation Details Lyophilized
Host Goat
Pack Size 2 mL
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.
Purity Purified
Solubility Soluble in water
Species Reactivity Mouse
Specificity The IgG fraction is tested for purity and specificity at 40 mg/ml using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected. This product shows reactivity to mouse complement C3; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present.
Typical Working Concentration Suggested working dilutions: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immunoblotting; 1:1,000 - 1:4,000 for ELISA
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)

Material Safety Data Sheet

Material Safety Data Sheet
English (United Kingdom)
Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United States)