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Anti-human complement C3 goat F(Ab')2 fragment, fluorescein-conjugated, 2 mL

Anti-human complement C3 goat F(Ab')2 fragment, fluorescein-conjugated, 2 mL

CN¥0.00

Key features and details

Lyophilized fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat F(ab')2 fragment to human complement C3 and buffer salts.

SKU: 0855191

Synonyms
Anti-C3; AHUS5; ARMD9; ASP; CPAMD1
Packaging size:
2 mL
Product Description

Fluorescein-Conjugated Goat F(ab')2 Fragment to Human Complement C3 is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat F(ab')2 fragment to human complement C3 and buffer salts.

Application Notes

Fluorescein-Conjugated Goat F(ab')2 Fragment to Human Complement C3 has been successfully used in immunohistochemistry (frozen) applications. It is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. It has also been used in flow cytometry studies. It has been used to assess the binding of complement factors.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Protein Purification| Flow Cytometry (FACS) | ELISA | Immunohistochemistry (Paraffin) | Immunoblot

Specifications
SKU 0855191
Alternate Names Anti-C3; AHUS5; ARMD9; ASP; CPAMD1
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-Conjugated Goat F(ab')2 Fragment to Human Complement C3 has been successfully used in immunohistochemistry (frozen) applications. It is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. It has also been used in flow cytometry studies. It has been used to assess the binding of complement factors.
Base Catalog Number 55191
Concentration Total Protein: 16.5 mg/mL; Antibody Protein: 7 mg/mL
Conjugate FITC
Formulation Details Lyophilized
Host Goat
Immunogen Human C3 purified from serum
Pack Size 2 mL
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.
Purity Purified
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human
Specificity This product shows reactivity to human complement C3; cross-reactivity to other species may exist.
Typical Working Concentration Immunohistochemistry (Frozen) (IHC (F)) 1:50 (dilution). Dilution Recommended: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA
Uniprot Number (Hu) P01024
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
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Certificates of Analysis

Certificates of Analysis
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Certificates of Analysis
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Certificates of Analysis
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Material Safety Data Sheet

Material Safety Data Sheet
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Material Safety Data Sheet
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Material Safety Data Sheet
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