还原型 β-烟酰胺腺嘌呤二核苷酸 二钠盐 水合物

还原型 β-烟酰胺腺嘌呤二核苷酸 二钠盐 水合物

Synonyms β-DPNH; β-NADH, 2Na; DPNH; Diphosphopyridine nucleotide reduced form; NADH ; Reduced DPN; Cozymase, reduced form; Dihydrophosphopyridine nucleotide, reduced form; Dihydronicotinamide adenine dinucleotide

CAS Number: 606-68-8 Molecular Formula: C21H27N7O14P2Na2 Molecular Weight: 709.409 g/mol

EC Number: 210-123-3 MDL Number: MFCD03791273

SKU 02101168-CF
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Description

Product Description

β-Nicotinamide Adenine Dinucleotide Reduced, Disodium Salt

Application Notes

β-Nicotinamide adenine dinucleotide (NAD+) and β-Nicotinamide adenine dinucleotide, reduced (NADH) comprise a coenzyme redox pair (NAD+:NADH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. In addition to its redox function, NAD+/NADH is a donor of ADP-ribose units in ADP-ribosylaton (ADP-ribosyltransferases; poly(ADP-ribose) polymerases ) reactions and a precursor of cyclic ADP-ribose (ADP-ribosyl cyclases). Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NADH as a cofactor and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. β-NADH is fluorescent; whereas, β-NAD+ is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10−7 M. Discussion of optimizing the fluorescence intensity and identification of interfering substances has been reported.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Coenzyme

Specifications
SKU 02101168-CF
Alternate Names β-DPNH; β-NADH, 2Na; DPNH; Diphosphopyridine nucleotide reduced form; NADH ; Reduced DPN; Cozymase, reduced form; Dihydrophosphopyridine nucleotide, reduced form; Dihydronicotinamide adenine dinucleotide
Application Notes β-Nicotinamide adenine dinucleotide (NAD+) and β-Nicotinamide adenine dinucleotide, reduced (NADH) comprise a coenzyme redox pair (NAD+:NADH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. In addition to its redox function, NAD+/NADH is a donor of ADP-ribose units in ADP-ribosylaton (ADP-ribosyltransferases; poly(ADP-ribose) polymerases ) reactions and a precursor of cyclic ADP-ribose (ADP-ribosyl cyclases). Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NADH as a cofactor and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. β-NADH is fluorescent; whereas, β-NAD+ is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10−7 M. Discussion of optimizing the fluorescence intensity and identification of interfering substances has been reported.
Base Catalog Number 101168
Biochemical Physiological Actions NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, beta-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease. As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.
CAS # 606-68-8
EC Number 210-123-3
Extinction Coefficient EmM = 6.22 (340 nm) and 14.4 (259 nm, pH 9.5)(Lit.)
Format Powder
Molecular Formula C21H27N7O14P2Na2
Molecular Weight 709.409 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Purity ≥98%
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance Water: λ max: 258 - 260 nm