Arachidonic acid

Arachidonic acid

Synonyms 5,8,11,14-Eicosatetraenoic Acid; Eicosa-5Z,8Z,11Z,14Z-tetraenoic acid; Immunocytophyte

CAS Number: 506-32-1 Molecular Formula: C20H32O2 Molecular Weight: 304.474 g/mol

Beilstein Registry Number: 1713889 EC Number: 208-033-4 MDL Number: MFCD00004417

SKU 02194625-CF
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Description

Product Description

Arachidonic Acid

Application Notes

Arachidonic Acid is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes. Arachidonic acid plays a key role in cellular regulation and is controlled through multiple interconnected pathways.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Precursor in the biosynthesis of prostaglandins; thromboxanes; and leukotrienes | cellular regulation

Specifications
SKU 02194625-CF
Alternate Names 5,8,11,14-Eicosatetraenoic Acid; Eicosa-5Z,8Z,11Z,14Z-tetraenoic acid; Immunocytophyte
Application Notes Arachidonic Acid is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes. Arachidonic acid plays a key role in cellular regulation and is controlled through multiple interconnected pathways.
Base Catalog Number 194625
Beilstein Registry Number 1713889
Biochemical Physiological Actions Arachidonic acid (AA) is an unsaturated ω6 fatty acid constituent of the phospholipids of cell membranes. Phospholipase A2 releases AA from the membrane phospholipids in response to inflammation. AA is subsequently metabolized to prostaglandins and thromboxanes by at least two cyclooxygenase (COX) isoforms, to leukotrienes and lipoxins by lipoxygenases, and to epoxyeicosatrienoic acids via cytochrome p450-catalyzed metabolism. AA and its metabolites play important roles in a variety of biological processes, including signal transduction, smooth muscle contraction, chemotaxis, cell proliferation and differentiation, and apoptosis. AA has been demonstrated to bind to the a subunit of G protein and inhibit the activity of Ras GTPase-activating proteins (GAPs). Cellular uptake of AA is energy dependent and involves protein-facilitated transport across the plasma membrane.
Boiling Point 170 °C at 1.50E-01 mm Hg
CAS # 506-32-1
EC Number 208-033-4
Format Liquid
Grade Cell Culture Grade
Hazard Statements May form explosive peroxides.
Melting Point -49.5 °C
Molecular Formula C20H32O2
Molecular Weight 304.474 g/mol
Personal Protective Equipment Eyeshields, Faceshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge
Preparation Method If ethanol is undesirable, arachidonic acid may be dissolved in acetonitrile, DMF, or DMSO. Simply evaporate the ethanol under a gentle stream of nitrogen (be certain not to evaporate the material to dryness) and redissolve the arachidonic acid in the solvent of choice.Just prior to use, make dilutions of the stock solution into aqueous buffer or isotonic saline to bring the arachidonic acid to the desired concentration. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiologic effects at low concentrations. A control using the solvent in the absence of the prostaglandin will address this potential variable. We do not recommend storing the aqueous solution for more than one day. It is difficult to obtain aqueous solutions of arachidonic acid directly. However, an organic solvent free solution of arachidonic acid can be prepared using concentrated basic buffers (pH > 8.0 and ionic strength not less than 0.1 M). Add 400 μL of cold buffer (0 °C) per mg of arachidonic acid and agitate vigorously and/or ultrasonicate.
Purity ≥98%
Refractive Index n20/D 1.4872(Lit.)
RTECS Number CE6675000
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.