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牛血清白蛋白, 低内毒素, 层析法, 干细胞级, ≥97%

牛血清白蛋白, 低内毒素, 层析法, 干细胞级, ≥97%

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CAS:9048-46-8 牛血清白蛋白第五组分;牛白蛋白;牛血清白蛋白Ⅴ 牛白蛋白是一条单条多肽链, 约由583至595个氨基酸残基组成, 不含碳水化合物, 包含17个链内二硫键和1个巯基。

SKU: 02199896-CF

CAS Number:
9048-46-8
Molecular Weight:
66KDa
EC Number:
232-936-2
Product Description

AlbumiNZ™低内毒素牛血清白蛋白由新西兰来源牛血浆通过符合ISO质量体系的色谱纯化工艺纯化获得,确保完整的可溯性和始终如一的高质量,具有极低的内毒素含量和极低的IgG水平,可简化下游纯化。符合9CFR(《美国联邦法规汇编》第9卷)要求,不含任何病毒,达到最高的生物安全水平。该款BSA可支持干细胞的生长并增加杂交瘤细胞的蛋白产量。

Application Notes

白蛋白结合水、钙离子、钠离子和钾离子。由于疏水基团的存在,白蛋白结合脂肪酸,胆红素,激素和药物。白蛋白的主要生物学功能是调节血液的胶体渗透压,并在较小程度上提供细胞营养。牛白蛋白含有16%的氮,常被用作蛋白质校准研究的标准。白蛋白用于溶解脂类,稳定蛋白质溶液,在Western blots或ELISA应用中也用作阻断剂。

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Stem cell research | Cell Culture applications | Immunoassay

Specifications
SKU 02199896-CF
Application Notes 白蛋白结合水、钙离子、钠离子和钾离子。由于疏水基团的存在,白蛋白结合脂肪酸,胆红素,激素和药物。白蛋白的主要生物学功能是调节血液的胶体渗透压,并在较小程度上提供细胞营养。牛白蛋白含有16%的氮,常被用作蛋白质校准研究的标准。白蛋白用于溶解脂类,稳定蛋白质溶液,在Western blots或ELISA应用中也用作阻断剂。
Base Catalog Number 199896
Biochemical Physiological Actions Albumin loses its helical structure rapidly in 2-4 M urea and 4 M guanidinium chloride. The unfolding is reversible, even, to some extent, after concomitant reduction of all disulfide bonds . Reduced albumin can reform into structures capable of binding antibodies and other ligands . Formation of SS bonds is rapid but the return of native structure is slow and some wrong disulfide pairings may persist . After oxidation of the cystine bridges, however, native structure is irreversibly lost.
CAS # 9048-46-8
Concentration Total Protein: ≥95%
EC Number 232-936-2
Format Lyophilized powder
Grade Cell Culture Grade, ImmunoAssay (EIA) Grade
Host Bovine
Molecular Weight 66KDa
pH 6.5 - 7.5 (4% aq soln)
Purity ≥97% Total Protein
Solubility Albumins are readily soluble in water and can only be precipitated by high concentrations of neutral salts such as ammonium sulfate. The solution stability of albumin is very good (especially if the solutions are stored aliquoted and frozen). Albumin is readily coagulated by heat. When heated to 50 °C or above, albumin quite rapidly forms hydrophobic aggregates which do not revert to monomers upon cooling. Dissolves completely in 20 min at 20-25 °C (20%).
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
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