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5-Bromo-4-chloro-3-indolyl phosphate, disodium salt

5-Bromo-4-chloro-3-indolyl phosphate, disodium salt

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5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt

SKU: 02150042-CF

Synonyms
BCIP; X-phosphate disodium salt
CAS Number:
102185-33-1
Molecular Formula:
C8H4BrClNO4PNa2
Molecular Weight:
370.43 g/mol
MDL Number:
MFCD00036757
Product Description

5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt

Application Notes

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP disodium salt is soluble in water. It may be used to prepare a stock solution, which in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Substrate for alkaline phosphatase

Specifications
SKU 02150042-CF
Alternate Names BCIP; X-phosphate disodium salt
Application Notes For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP disodium salt is soluble in water. It may be used to prepare a stock solution, which in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color.
Base Catalog Number 150042
CAS # 102185-33-1
Format Powder
Molecular Formula C8H4BrClNO4PNa2
Molecular Weight 370.43 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Purity ≥99%
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance λ max (water): 291 ± 3 nm
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