4-Thiouridine

4-Thiouridine

CAS Number: 13957-31-8 Molecular Weight: 260.264 g/mol

EC Number: 237-735-3 MDL Number: MFCD00006538

SKU 02152134-CF
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Description

Product Description

4-Thiouridine

Application Notes

4-Thio Uridine is typically used to modify oligos slated for RNA, or RNA-protein, structural studies. A 4-thio-rU modified RNA pentamer was used to study the effect of this modification on codon-anticodon interaction when it is in the wobble position of tRNA. Because 4-thio-rU is photoreactive, 4-thio-rU modified RNA oligos have also been used as photoaffinity probes in the role of substrate analogs for characterizing the enzyme:substrate complex of tRNA:pseudouridine-5S synthase . 4-thio-rU modified oligos have also been used as modules for assembling U25 small nucleolar RNAs (U25snoRNA) by ligation. These snoRNAs were used in cross-linking studies to identify which proteins assembled on them in vivo in Xenopus oocytes. In addition, because the thiol group is chemically reactive, other moieties can be conjugated at the thiol group of 4-thio-rU. Such a strategy was used to introduce spin labels to 4-thio-rU-containing RNA oligos.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Antisense agent | Uridine analogue | Study the specificity and kinetics of uridine-cytidine kinases

Specifications
SKU 02152134-CF
Application Notes 4-Thio Uridine is typically used to modify oligos slated for RNA, or RNA-protein, structural studies. A 4-thio-rU modified RNA pentamer was used to study the effect of this modification on codon-anticodon interaction when it is in the wobble position of tRNA. Because 4-thio-rU is photoreactive, 4-thio-rU modified RNA oligos have also been used as photoaffinity probes in the role of substrate analogs for characterizing the enzyme:substrate complex of tRNA:pseudouridine-5S synthase . 4-thio-rU modified oligos have also been used as modules for assembling U25 small nucleolar RNAs (U25snoRNA) by ligation. These snoRNAs were used in cross-linking studies to identify which proteins assembled on them in vivo in Xenopus oocytes. In addition, because the thiol group is chemically reactive, other moieties can be conjugated at the thiol group of 4-thio-rU. Such a strategy was used to introduce spin labels to 4-thio-rU-containing RNA oligos.
Base Catalog Number 152134
CAS # 13957-31-8
EC Number 237-735-3
Format Powder
Molecular Weight 260.264 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Purity ~98%
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.