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3-(4,5-Dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide, cell culture reagent

3-(4,5-Dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide, cell culture reagent

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3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide

SKU: 02194592-CF

Synonyms
MTT; Thiazolyl Blue;3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide;Methylthiazolyldiphenyl-tetrazolium bromide
CAS Number:
298-93-1
Molecular Formula:
C18H16BrN5S
Molecular Weight:
414.325 g/mol
Beilstein Registry Number:
4081397
EC Number:
206-069-5
MDL Number:
MFCD00011964
Product Description

3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide

Application Notes

3-(4,5-Dimethyl-2-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide is used as a colorimetric metabolic activity indicator in cell viability assays. Thiazolyl Blue Tetrazolium Blue (MTT) is a dye used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Colorimetric assay; Cell viability; cell culture

Specifications
SKU 02194592-CF
Alternate Names MTT; Thiazolyl Blue;3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide;Methylthiazolyldiphenyl-tetrazolium bromide
Application Notes 3-(4,5-Dimethyl-2-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide is used as a colorimetric metabolic activity indicator in cell viability assays. Thiazolyl Blue Tetrazolium Blue (MTT) is a dye used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Base Catalog Number 194592
Beilstein Registry Number 4081397
CAS # 298-93-1
EC Number 206-069-5
Grade Cell Culture Grade
Hazard Statements H315-H319-H335-H341
Melting Point 195 deg C (decomposes)
Molecular Formula C18H16BrN5S
Molecular Weight 414.325 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Purity ~98%
RTECS Number XF8060000
Safety Symbol GHS07, GHS08
Solubility Solubility in <a class="pubchem-internal-link CID-8019" href="https://pubchem.ncbi.nlm.nih.gov/compound/2-methoxyethanol">2-methoxyethanol</a>, 20 mg/mL; <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>, 20 mg/mL
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance 376 ± 2 nm; 242 ± 2 nm λmax (methanol)
Vapor Pressure 5.11X10-11 mm Hg at 25 deg C /Estimated/
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