噻唑蓝(MTT)

噻唑蓝(MTT)

Synonyms MTT; Thiazolyl Blue Tetrazolium Bromide; Methylthiazolyldiphenyl-tetrazolium bromide

CAS Number: 298-93-1 Molecular Formula: C18H16BrN5S Molecular Weight: 414.325 g/mol

Beilstein Registry Number: 4081397 EC Number: 206-069-5 MDL Number: MFCD00011964

SKU 02102227-CF
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Description

Product Description

3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide

Application Notes

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Histochemical/Cytochemical Reagent

Specifications
SKU 02102227-CF
Alternate Names MTT; Thiazolyl Blue Tetrazolium Bromide; Methylthiazolyldiphenyl-tetrazolium bromide
Application Notes Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Base Catalog Number 102227
Beilstein Registry Number 4081397
CAS # 298-93-1
EC Number 206-069-5
Hazard Statements H315-H319-H335-H341
Melting Point 195 deg C (decomposes)
Molecular Formula C18H16BrN5S
Molecular Weight 414.325 g/mol
Personal Protective Equipment Eyeshields, Faceshields, Gloves, respirator cartridges
Purity ~98%
RTECS Number XF8060000
Safety Symbol GHS07,GHS08
Solubility Solubility in <a class="pubchem-internal-link CID-8019" href="https://pubchem.ncbi.nlm.nih.gov/compound/2-methoxyethanol">2-methoxyethanol</a>, 20 mg/mL; <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>, 20 mg/mL
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance 376 ± 2 nm; 242 ± 2 nm
Vapor Pressure 5.11X10-11 mm Hg at 25 deg C /Estimated/