The FastProtein Blue Matrix is optimized for the rapid lysis of both gram positive and gram negative bacteria. Cells are resuspended in either 1X PBS or other expression buffer and added to the Lysing Matrix B tube (0.1mm silica beads). After 20-40 seconds processing in a FastPrep® Instrument or equivalent bead-beater, proteins can be extracted from the centrifuged homogenate using a wide variety of methods.
Designed for extraction of protein from bacterial cultures.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Protein isolation from bacteria.
|Application Notes||Designed for extraction of protein from bacterial cultures.|
|Base Catalog Number||6550400,6550500|
|Components||Contains 2mL tubes of Lysing Matrix B which contain 0.1mm silica (glass) and protocol for protein isolation of bacteria.|
|FastPrep Instruments Models||ConeFlex™ Legacy (allows all FastPrep-24™ adapters to be used on the FastPrep-96™.), CoolPrep™ (24x2 mL), HiPrep™ (48x2 mL), QuickFlex™ (96x2 mL), QuickPrep™ (24x2 mL)|
|Personal Protective Equipment||If working with biological, toxic, hazardous or infectious samples use hand protection (latex, nitrile or other plastic material gloves), skin and body protection (labcoat and sleeves) and eye protection (safety glasses with side-shields). Handle in accordance with good industrial hygiene and safety practice.|
|Sample Volume||up to 200 mg per prep|