EMM no nitrogen (no NH4CL, glutamate), 227 g

EMM no nitrogen (no NH4CL, glutamate), 227 g

Synonyms Edinburgh's Minimal Medium No Nitrogen |S.pombe growth medium

Packaging size: 227 g

SKU 114110712
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Description

Product Description

EMM Nitrogen (No NH4Cl, Glutamate) is a minimal defined medium for the growth of S. pombe, containing dextrose, buffers, salts, vitamins and trace minerals. EMM minimal defined medium is thiamine-free nmt1 promoter studies in Schizosaccharomyces pombe.

Application Notes

EMM w/o Nitrogen is used for the growth of S.pombe.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. pombe Culture

Specifications
SKU 114110712
Alternate Names Edinburgh's Minimal Medium No Nitrogen |S.pombe growth medium
Application Notes EMM w/o Nitrogen is used for the growth of S.pombe.
Base Catalog Number 4110712
Format Powder
Formulation Details Contents Per Liter: 20 g d-glucose; 3 g potassium hydrogen phthalate (14.7 mM); 2.2 g Na2HPO4 (15.5 mM); 1.05 g MgCl2.6H2O (5.2 mM); 14.7 µg CaCl2.2H2O (99.8 µM); 1 g KCl (13.4 mM); 0.04 g Na2SO4 (0.28 mM); 1mg pantothenic acid (4.2 µM); 10 mg nicotinic acid (81.2 µM); 10 mg myo-inositol (55.5 µM); 10 µg biotin (0.82 µM); 0.5 mg boric acid (8.9 µM); 0.4 mg MnSO4 (2.4 µM); 0.4 mg ZnSO4.7H2O (1.4 µM); 0.2 mg FeCl2.6H2O (0.7 µM); 40 µg molybdic acid (0.25 µM); 0.1 mg KI (0.6 µM); 40 µg CuSO4.5H2O (0.016 µM); 1 mg citric acid (4.76 µM).
Grade Molecular Biology Certified™
Media Type EMM
Pack Size 227 g
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice
pH pH Range: 5.4 - 6.4 at 25 °C
Preparation Method Reagents are tested and chosen for their ability to promote optimum growth of S. pombe when combined in EMM Broth formulations. Chosen reagents are combined in the proper proportions according to the formulation listed above and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare agar medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.
Solubility Soluble in water (27 g/L).
Sterilization of Solutions Autoclaved at 121 °C for 15 minutes. Note: Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not normally affect growth of cells, only the appearance of the sterile medium).
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.