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5-FOA (5-fluoroorotic acid), 1 g

5-FOA (5-fluoroorotic acid), 1 g

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5-Fluoroorotic Acid monohydrate is a derivative of a pyrimidine precursor and is selectively toxic to yeast cells which synthesize orotidine-5’-phosphate decarboxylase.

SKU: 114066102

Synonyms
2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid; 5-Fluorouracil-4-carboxylic acid; 5-Fluoroorotate; Fluoroorotic Acid; FOA; 4-Pyrimidinecarboxylic Acid; 5-Fluoro-1,2,3,6-Tetrahydro-2,6-Dioxo-(9CI); 1,2,3,6-Tetrahydro-2,6-Dioxo-5-Fluoro-4-Pyrimidinecarboxylic Acid; 5-Fluoro-6-Carboxyuracil
CAS Number:
703-95-7
Molecular Formula:
C5H3FN2O4·H2O
Molecular Weight:
174.09 (anhydrous)
EC Number:
211-876-0
Packaging size:
1 g
Product Description

5-Fluoroorotic Acid monohydrate is a derivative of a pyrimidine precursor and is selectively toxic to yeast cells which synthesize orotidine-5’-phosphate decarboxylase.

Application Notes

5-Fluoroorotic Acid monohydrate (FOA) is used as a selective agent in yeast molecular genetics. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. It is also used in the selection of Ura cells in a population of Ura+ cells; and in the selection of orotidine-5’-phosphate decarboxylase (OMPdecase) mutants of Saccharomyces cerevisiae. FOA (0.1 mg/mL) has been used in the positive selection for uracil auxotrophs of the sulfur-dependent thermophilic archaebacterium Sulfolobus acidocaldarius. It is a noncompetitive inhibitor of dihydroorotase. FOA (50% inhibition, 6 nM) was a selective inhibitor of malarial cells of Plasmodium falciparum in vitro and in vivo. FOA showed anti-tumor activity against transplanted tumors in rats and mice; a bacteriostatic effect in vitro against various microorganisms, particularly gram-negative bacteria; antimycotic activity against various types of mold.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture

Specifications
SKU 114066102
Alternate Names 2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid; 5-Fluorouracil-4-carboxylic acid; 5-Fluoroorotate; Fluoroorotic Acid; FOA; 4-Pyrimidinecarboxylic Acid; 5-Fluoro-1,2,3,6-Tetrahydro-2,6-Dioxo-(9CI); 1,2,3,6-Tetrahydro-2,6-Dioxo-5-Fluoro-4-Pyrimidinecarboxylic Acid; 5-Fluoro-6-Carboxyuracil
Application Notes 5-Fluoroorotic Acid monohydrate (FOA) is used as a selective agent in yeast molecular genetics. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. It is also used in the selection of Ura cells in a population of Ura+ cells; and in the selection of orotidine-5’-phosphate decarboxylase (OMPdecase) mutants of Saccharomyces cerevisiae. FOA (0.1 mg/mL) has been used in the positive selection for uracil auxotrophs of the sulfur-dependent thermophilic archaebacterium Sulfolobus acidocaldarius. It is a noncompetitive inhibitor of dihydroorotase. FOA (50% inhibition, 6 nM) was a selective inhibitor of malarial cells of Plasmodium falciparum in vitro and in vivo. FOA showed anti-tumor activity against transplanted tumors in rats and mice; a bacteriostatic effect in vitro against various microorganisms, particularly gram-negative bacteria; antimycotic activity against various types of mold.
Base Catalog Number 4066102
CAS # 703-95-7
EC Number 211-876-0
Format Powder
Grade Molecular Biology Certified™
Media Type Attachments Factors
Melting Point 278 °C (dec.) (Lit.)
Molecular Formula C5H3FN2O4·H2O
Molecular Weight 174.09 (anhydrous)
Pack Size 1 g
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice
Safety Symbol GHS07
Solubility Soluble in 4 M NH4OH (50 mg/mL)(Sonication or heating may be needed), slightly soluble in water.
Typical Working Concentration 1 mg/mL has been used as a selective agent in yeast molecular genetics
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

Certificates of Analysis

Certificates of Analysis
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Certificates of Analysis
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Certificates of Analysis
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Certificates of Analysis
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Certificates of Analysis
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Material Safety Data Sheet

Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United States)