SOB media is a standard media the preparation of cells for chemical transformation.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Medium used in preparing Escherichia coli competent cells for transformation
|Alternate Names||Competent Cell Media for Bacteria|
|Base Catalog Number||3030021|
|Formulation Details||Typically contains per liter: 20 g Tryptone-B; 5 g Yeast Extract-B; 0.5 g NaCl; 5 g MgSO4∙7H2O. Small quantities of biological buffers have been added for proper pH adjustment.|
|Grade||Molecular Biology Certified™|
|Pack Size||454 g|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|pH||Acceptable pH range is 6.4-7.5 at 25 °C. Please note that the pH of SOB Medium liquid increases as the temperature of the solution decreases.|
|Preparation Method||Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in SOB formulations. Chosen reagents are combined in the proper proportions according to the SOB formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid medium by adding 31 g of powder per liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve and autoclaved at 121°C for 15 minutes. Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not noticeably affect growth of cells, only the appearance of the sterile medium).|
|Solubility||Soluble in water (31 g/L).|
|Usage Statement||Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.|