Anti-Actin, mouse monoclonal antibody (Clone C4)

Anti-Actin, mouse monoclonal antibody (Clone C4)

Synonyms Monoclonal Anti-Actin

SKU 0869100-CF
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Description

Product Description

The monoclonal actin (clone C4) is a mouse IgG1 (gamma 1 heavy/kappa light chain) antibody. The monoclonal actin (clone C4) is a mouse IgG1 (gamma 1 heavy/kappa light chain) antibody. Chicken gizzard actin was used as the immunogen. Immunoblots demonstrate specificity directed towards all actins ranging from yeast, to flies, to mouse, to man. These are skeletal alpha-actin, cardiac alpha-actin, smooth muscle gamma actin, cytoplasmic beta-actin, cytoplasmic gamma actin as well as Dictyostelium discoideum and Physarum olycephalum actins. The epitope for the antibody lies between residues 18-40, a highly conserved region of the molecule. This antibody can also be used for cells cultivated on slides, for frozen tissue sections and as a loading control for western blots.

Application Notes

Actin Monoclonal Antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading. Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody. Also It's used in Indirect ELISA, Immunofluorescence, Immunoblots and Immunohistochemistry.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

ELISA | Immunohistochemistry | Immunoblot | Immunofluorescence assays (IFA)

Specifications
SKU 0869100-CF
Alternate Names Monoclonal Anti-Actin
Antibody Type Monoclonal Antibody
Application Notes Actin Monoclonal Antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading. Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody. Also It's used in Indirect ELISA, Immunofluorescence, Immunoblots and Immunohistochemistry.
Base Catalog Number 691001,691002
Clone Name C4
Conjugate Unconjugated
Formulation Details Liquid
Host Mouse
Isotype IgG1, kappa
Personal Protective Equipment Eyeshields, Gloves, half-mask respirator , multi-purpose combination respirator cartridge
Solubility Soluble in water
Specificity Immunoblots demonstrate specificity directed towards all six known vertebrate isoactins as well as Dictyostelium discoideum and Physarum polycephalum actins
Typical Working Concentration Indirect Immunofluorescence: 1:500 Immunoblots: 1:500 for DAB/peroxidase detection, actin band migrates at 42 kDa on SDS-PAGE. Immunohistochemistry: 1:500
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.