Anti-sheep red blood cells rabbit IgG fraction, 2 mL

Anti-sheep red blood cells rabbit IgG fraction, 2 mL

Packaging size: 2 mL

SKU 0855806
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Description

Product Description

Product is the lyophilized powder of rabbit IgG fraction to sheep red blood cells and buffer salts.

Application Notes

Immunoblotting; Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. This product is suitable for sensitizing cells in hemolytic complement assays, and for lymphocyte screening procedures, especially screening for T cells and T receptor-bearing lymphocytes. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | immmunoelectrophoresis| Immunohistochemistry (Paraffin; acetone)

Specifications
SKU 0855806
Antibody Type Polyclonal Antibody
Application Notes Immunoblotting; Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. This product is suitable for sensitizing cells in hemolytic complement assays, and for lymphocyte screening procedures, especially screening for T cells and T receptor-bearing lymphocytes. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers.
Base Catalog Number 55806
Concentration Total Protein: 20.5 mg/mL Hemagglutination Titer: 1:1600
Conjugate Unconjugated
Formulation Details Lyophilized
Host Rabbit
Pack Size 2 mL
Preparation Method The antiserum is prepared by immunizing healthy rabbits with red blood cells obtained from healthy sheep. The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material
Species Reactivity Sheep
Specificity The total protein is measured using the Biuret procedure; with bovine albumin as a standard. Hemagglutination titer is standardized with an in-house control by measurement of agglutination end-point with 10% sheep red blood cells. Each IgG fraction is tested for purity at a minimum of 40 mg/ml using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.