Anti-rat albumin sheep IgG fraction, peroxidase-conjugated, 2 mL

Anti-rat albumin sheep IgG fraction, peroxidase-conjugated, 2 mL

MDL Number: MFCD00162813

Packaging size: 2 mL

SKU 0855776
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Description

Product Description

Peroxidase-conjugated sheep IgG fraction to rat albumin is the lyophilized powder of horseradish peroxidase (HRP)-conjugated sheep IgG fraction to rat albumin and buffer salts. IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG conjugated to a detectable substrate is a useful tool for the analysis of target proteins. Anti-Rat IgG (whole molecule)-Peroxidase antibody binds all rat immunoglobulins.

Application Notes

Peroxidase-conjugated sheep IgG fraction to rat albumin is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Lysates generated from primary cells isolated from lymphoid organs of P. monodon shrimp were analyzed by western blot using HRP-conjugated goat anti-rat as the secondary antibody at a 1:4000 dilution.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | ELISA | Enzyme Immunoassays | Immunoblot

Specifications
SKU 0855776
Antibody Type Polyclonal Antibody
Application Notes Peroxidase-conjugated sheep IgG fraction to rat albumin is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Lysates generated from primary cells isolated from lymphoid organs of P. monodon shrimp were analyzed by western blot using HRP-conjugated goat anti-rat as the secondary antibody at a 1:4000 dilution.
Base Catalog Number 55776
Concentration Total Protein: 20.8 mg/mL Titer: 1:109;000
Conjugate HRP
Formulation Details Lyophilized
Host Sheep
Immunogen Purified rat IgG
Pack Size 2 mL
Preparation Method Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative has been added.
Purity Purified
Solubility Soluble in water
Species Reactivity Rat
Specificity It shows reactivity to rat albumin; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present.
Typical Working Concentration SUGGESTED WORKING DILUTIONS: 1:500 - 1:4,000 for Tissue Staining 1:1,000 - 1:8,000 for Immunoblotting 1:2,000 - 1:16,000 for Elisa
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.