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Anti-mouse IgG (whole molecule) affinity-purified goat F(Ab')2 fragment, 2 mg

Anti-mouse IgG (whole molecule) affinity-purified goat F(Ab')2 fragment, 2 mg

CN¥0.00
Goat affinity purified F(ab')2 fragments to mouse IgG (whole molecule) and buffer salts.

SKU: 0855487

MDL Number:
MFCD00162638
Packaging size:
2 mg
Product Description

Product is goat affinity purified F(ab')2 fragments to mouse IgG (whole molecule) and buffer salts.

Application Notes

Goat Affinity Purified F(ab')2 Fragment to Mouse IgG (Whole Molecule) is used as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. Used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Precipitating antibody |Immunoprecipitation

Specifications
SKU 0855487
Antibody Type Polyclonal Antibody
Application Notes Goat Affinity Purified F(ab')2 Fragment to Mouse IgG (Whole Molecule) is used as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. Used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Base Catalog Number 55487
Concentration Total Protein: 1.2 mg/mL; Active Antibody: 1.0 mg/mL
Conjugate Unconjugated
Formulation Details Purified
Host Goat
Immunogen IgG purified from pooled normal mouse serum
Pack Size 2 mg
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Preparation Method The affinity purified F(ab')<sub>2</sub> fragment is prepared from the specific goat IgG fraction by limited digestion with pepsin and solid phase absorption. The resulting affinity purified F(ab')<sub>2</sub> fragment is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.
Purity Affinity Purified
Solubility Soluble in water
Species Reactivity Mouse (Whole Molecule)
Specificity It shows reactivity to mouse IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
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