Anti-mouse IgG (whole molecule) goat IgG fraction, 2 mL

Anti-mouse IgG (whole molecule) goat IgG fraction, 2 mL

MDL Number: MFCD00162638

Packaging size: 2 mL

SKU 0855455
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Description

Product Description

Product is the lyophilized powder of goat IgG fraction to mouse IgG (whole molecule) and buffer salts.

Application Notes

Goat IgG fraction to mouse IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. The antibody was used in ELISA and RIA in various studies. It is also suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed) | Immunofluorescence assays (IFA)

Specifications
SKU 0855455
Antibody Type Polyclonal Antibody
Application Notes Goat IgG fraction to mouse IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. The antibody was used in ELISA and RIA in various studies. It is also suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).
Base Catalog Number 55455
Concentration Total Protein: 24.6 mg/mL; Antibody Protein: 10 mg/mL
Conjugate Unconjugated
Formulation Details Lyophilized
Host Goat
Immunogen Pooled normal mouse serum
Pack Size 2 mL
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Preparation Method The IgG fraction is prepared from the specific goat antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
Solubility Soluble in water
Species Reactivity Mouse (Whole Molecule)
Specificity It shows reactivity to mouse IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Typical Working Concentration Anti-Mouse IgG (whole molecule) antibody was used as secondary antibody in immunoblotting at a working dilution of 1:1000.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.