Anti-human IgG (whole molecule) goat IgG fraction, fluorescein-conjugated, 2 mL

Anti-human IgG (whole molecule) goat IgG fraction, fluorescein-conjugated, 2 mL

MDL Number: MFCD00162487

Packaging size: 2 mL

SKU 0855144
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Description

Product Description

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human IgG (whole molecule) and buffer salts.

Application Notes

This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855144
Antibody Type Polyclonal Antibody
Application Notes This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals.
Base Catalog Number 55144
Concentration Total Protein: 20.7 mg/mL; Antibody Protein: 10 mg/mL
Conjugate FITC
Formulation Details Lyophilized
Host Goat
Immunogen Pooled normal human serum
Pack Size 2 mL
Personal Protective Equipment Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide,adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.
Purity Purified
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human (Whole Molecule)
Specificity This product shows reactivity to human IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Sterility Filtered through a .22 µm filter
Typical Working Concentration Suggested working dilution: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.