IgA (Lambda2) MOPC 315, mouse myeloma, purified, 1 mL

IgA (Lambda2) MOPC 315, mouse myeloma, purified, 1 mL

MDL Number: MFCD00162614

Packaging size: 1 mL

SKU 0850325
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Description

Product Description

Mouse IgA (l2) is purified from clarified MOPC 315 mouse ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M Tris, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

Application Notes

This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control. It is suitable for Ouchterlony Double Diffusion. The antibody was used to detect antigens to Helicobacter hepaticus in mice serum using a concentration of 5 μg/mL.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Ouchterlony Double Diffusion

Specifications
SKU 0850325
Antibody Type Monoclonal Antibody
Application Notes This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control. It is suitable for Ouchterlony Double Diffusion. The antibody was used to detect antigens to Helicobacter hepaticus in mice serum using a concentration of 5 μg/mL.
Base Catalog Number 50325
Clone Name MOPC 315
Concentration 1.1 mg/mL
Conjugate Unconjugated
Formulation Details Purified
Host Mouse
Isotype IgA
Pack Size 1 mL
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Preparation Method Mouse IgA (λ2) is purified from clarified MOPC 315 mouse ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M Tris, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.
Purity Affinity Purified
Solubility Soluble in Water
Specificity The total protein is determined using the Biuret procedure with bovine albumin as the standard and a phosphate blank. The product contains 1 mg of mouse IgA. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgA; no trace of IgG; IgM or non-immunoglobulin serum proteins is detected.
Sterility Filtered through a .22 µm filter
Typical Working Concentration Detection of antigens to Helicobacter hepaticus in mice serum using a concentration of 5 μg/mL .
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.