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Proline Specific Endopeptidase (From Flavobacterium Meningosepticum), 50 U

Proline Specific Endopeptidase (From Flavobacterium Meningosepticum), 50 U

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Proline Specific Endopeptidase specifically cleaves peptide bonds on the carboxy side of proline residues. Much slower hydrolysis is observed on the carboxy side of alanine residues. This enzyme is very close, in its properties, to a post-proline cleaving enzyme. The substrates have been found to have the general structure Y-Pro-X, where Y is a peptide or N-protected amino acid and X may be an amino acid, peptide, amide or ester.

SKU: 08320821

Synonyms
Prolyl endopeptidase; Post-proline cleaving enzyme; Post-proline endopeptidase
CAS Number:
72162-84-6
Molecular Weight:
~78 kDa
Packaging size:
50 U
Product Description

Proline Specific Endopeptidase specifically cleaves peptide bonds on the carboxy side of proline residues. Much slower hydrolysis is observed on the carboxy side of alanine residues. This enzyme is very close, in its properties, to a post-proline cleaving enzyme. The substrates have been found to have the general structure Y-Pro-X, where Y is a peptide or N-protected amino acid and X may be an amino acid, peptide, amide or ester.

Application Notes

Proline specific endopeptidase is useful for the determination of amino acid sequences of peptides and proteins containing proline residues. Proline specific endopeptidases may be useful in cancer, neurology and diabetes research. Prolyl endopeptidase (PEP) may be used as a drug target for neuropsychiatric diseases such as stress disorder, depression, and schizophrenia.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Cloning Expression | Determination of amino acid sequences of peptides and proteins containing proline residues

Specifications
SKU 08320821
Alternate Names Prolyl endopeptidase; Post-proline cleaving enzyme; Post-proline endopeptidase
Application Notes Proline specific endopeptidase is useful for the determination of amino acid sequences of peptides and proteins containing proline residues. Proline specific endopeptidases may be useful in cancer, neurology and diabetes research. Prolyl endopeptidase (PEP) may be used as a drug target for neuropsychiatric diseases such as stress disorder, depression, and schizophrenia.
Base Catalog Number 320821
CAS # 72162-84-6
Formulation Details Lyophilized
Isoelectric Point 9.1
Molecular Weight ~78 kDa
Pack Size 50 U
pH Optimum pH 7.0
Protein or Enzyme Type Molecular Biology Enzymes
Source Flavobacterium meningosepticum
Specific Activity Not less than 5.0 U/mg material
Unit Definition One unit causes the formation of one μmole of p-nitroaniline per minute under specific pH and temperature conditions. Enzyme Activity: One unit is the enzyme activity which produces 1 μmol p-nitroaniline per minute at 30 °C, pH 7.0, from Z-Gly-Pro-pNA. (Z: carbobenzoxy-)
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)

Datasheets

Datasheets
English (United States)