DL-二硫苏糖醇(DTT)

DL-二硫苏糖醇(DTT)

Synonyms 1,4-Dithiothreitol; DTT; 1,4-Dithio-DL-threitol; Cleland Reagent; (±)-threo-1,4-Dimercapto-2,3-butanediol

CAS Number: 27565-41-9 Molecular Formula: C4H10O2S2

Beilstein Registry Number: 1719757 EC Number: 222-468-7 MDL Number: MFCD00004877

SKU 04856126-CF
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Description

Product Description

Dl-Dithiothreitol

Application Notes

Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Sulfhydryl reducing agent | Blotting | Protein Purification

Specifications
SKU 04856126-CF
Alternate Names 1,4-Dithiothreitol; DTT; 1,4-Dithio-DL-threitol; Cleland Reagent; (±)-threo-1,4-Dimercapto-2,3-butanediol
Application Notes Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Base Catalog Number 856126
Beilstein Registry Number 1719757
CAS # 27565-41-9
EC Number 222-468-7
Format Powder
Grade Electrophoresis grade
Half Life 1.4( pH 8.5 at 2 °C)
Hazard Statements H302-H315-H319-H335
Molecular Formula C4H10O2S2
Personal Protective Equipment Dust mask , Eyeshields, Faceshields, Gloves
Purity ≥99.5%
RTECS Number EK1610000
Safety Symbol GHS07
Typical Working Concentration Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.