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Chloroform

Chloroform

CN¥0.00
Chloroform

SKU: 02193814-CF

Synonyms
Formyl trichloride; Methane trichloride; Methenyl trichloride; NCI-C02686; RCRA Waste Number U044; TCM; Trichloroform; Trichloromethane
CAS Number:
67-66-3
Molecular Formula:
CHCl3
Molecular Weight:
119.369 g/mol
Beilstein Registry Number:
1731042
EC Number:
200-663-8
MDL Number:
MFCD00000826
Product Description

Chloroform

Application Notes

Chloroform is typically used as a solvent and as a cleansing agent. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Used for PCR aqueous phase recovery overlaid with mineral oil | Solvent | Cleansing agent | Chromatography

Specifications
SKU 02193814-CF
Alternate Names Formyl trichloride; Methane trichloride; Methenyl trichloride; NCI-C02686; RCRA Waste Number U044; TCM; Trichloroform; Trichloromethane
Application Notes Chloroform is typically used as a solvent and as a cleansing agent. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry.
Auto Ignition Not flammable (USCG, 1999)
Base Catalog Number 193814
Beilstein Registry Number 1731042
Boiling Point 143° F at 760 mm Hg (EPA, 1998)
CAS # 67-66-3
Density 1.4832 at 68° F (EPA, 1998)
EC Number 200-663-8
Flash Point None (EPA, 1998)
Format Liquid
Grade ACS grade
Hazard Statements H302-H315-H351-H373
Melting Point -82.3° F (EPA, 1998)
Molecular Formula CHCl3
Molecular Weight 119.369 g/mol
Personal Protective Equipment Eyeshields, Faceshields, full-face respirator, Gloves, multi-purpose combination respirator cartridge
Refractive Index n20/D 1.445(lit.)
RTECS Number FS9100000
Safety Symbol GHS07, GHS08
Solubility less than 1 mg/mL at 66° F (NTP, 1992)
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance λ: 245 nm Amax: 1.00, λ: 255 nm Amax: 0.25, λ: 260 nm Amax: 0.15, λ: 270 nm Amax: 0.05, λ: 290-400 nm Amax: 0.01(Lit.)
Vapor Density 4.12 (EPA, 1998) (Relative to Air)
Vapor Pressure 100 mm Hg at 50.72° F (EPA, 1998)
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