Specifications
| SKU | 0219020201 |
| Alternate Names | 2,7-Diamino-10-ethyl-9-phenyl- phenanthridinium bromide; Homidium bromide; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide; EtBr; Dromilac |
| Application Notes | Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. Confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. Subsequent fluorescence techniques enable the extraction and characterization of DNA bands. |
| Base Catalog Number | 19020201 |
| Beilstein Registry Number | 3642536 |
| Biochemical Physiological Actions | Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells. |
| CAS # | 1239-45-8 |
| EC Number | 214-984-6 |
| Hazard Statements | H302-H330-H341 |
| Melting Point | 260-262 °C (dec.) (Lit.) |
| Molecular Formula | C21H20N3Br |
| Molecular Weight | 394.3 |
| Pack Size | 1 g |
| Personal Protective Equipment | Eyeshields, Faceshields, full-face particle respirator, Gloves, respirator cartridge, respirator filter |
| RTECS Number | SF7950000 |
| Safety Symbol | GHS06, GHS08 |
| Solubility | Soluble in water (10mg/mL), Slightly soluble in ethanol; partially soluble in chloroform. |
| Typical Working Concentration | Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/mL and visualized immediately after electrophoresis. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | λmax = 475 nm; 520 nm (methanol), 480 nm (water); 515 (glycerol); 520 nm (acetone); 532 nm (ethanol); 535 (DMSO) and 540 nm (pyridine) (Lit.) |
Documents
Certificate of Analysis (COA)
![MP COA [0219020201] lot ID: U1122294698](https://www.mpbio.com/static/version1745479238/frontend/Mpbio/default/en_US/images/document.png "MP COA [0219020201] lot ID: U1122294698")
Datasheet
Datasheet
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