Peroxidase, ≥250 U/mg solid
SKU: 02195373-CF
Peroxidase
Application Notes
Horseradish peroxidase is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. It is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. HRP labeled immunoglobulins are used as probes for the demonstration of tissue antigens and in enzyme immunoassay (EIA) systems for determination of soluble and insoluble antigens. HRP is the most desired label for antibodies since it is the smallest and most stable of the three most popular enzyme labels (HRP, alkaline phosphatase, and B–galactosidase) and its glycosylation leads to lower non–specific binding. It is also useful for tracing neural connections. These include motor and sensory innervation of peripheral organs, and connections of peripheral nerves, ganglia and individual dorsal roots. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross–linkers.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
ELISA | Immunoblotting | Immunohistochemistry
| SKU | 02195373-CF |
| Alternate Names | HRP; Donor:hydrogen-peroxide oxidoreductase; E.C. 1.11.1.7; Horseradish peroxidase; Peroxidase from horseradish |
| Application Notes | Horseradish peroxidase is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. It is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. HRP labeled immunoglobulins are used as probes for the demonstration of tissue antigens and in enzyme immunoassay (EIA) systems for determination of soluble and insoluble antigens. HRP is the most desired label for antibodies since it is the smallest and most stable of the three most popular enzyme labels (HRP, alkaline phosphatase, and B–galactosidase) and its glycosylation leads to lower non–specific binding. It is also useful for tracing neural connections. These include motor and sensory innervation of peripheral organs, and connections of peripheral nerves, ganglia and individual dorsal roots. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross–linkers. |
| Base Catalog Number | 195373 |
| Biochemical Physiological Actions | When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification. HRP reacts with hydrogen peroxide to form a [HRP-H2O2] complex which can oxidize a wide variety of hydrogen donors. |
| CAS # | 9003-99-0 |
| EC Number | 232-668-6 |
| Extinction Coefficient | 100 (403 nm)(Lit.) |
| Format | Crystalline powder |
| Isoelectric Point | 7.2(Lit.); Isozymes range from 3.0–9.0(Lit) |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Source | Horseradish(Amoracia rusticana) |
| Specific Activity | ≥250 u/mg solid |
| Specificity | Activity is observed with H2O2; MeOOH and EtOOH. |
| Typical Working Concentration | For assay of rate of decomposition hydrogen peroxidide by peroxidase with pyrogallol: 0.50% (w/w) Hydrogen peroxide solution (H2O2); Prepare 50 mL in deionized water using a 30% Hydrogen peroxide solution. RZ Determination=0.5 mg/mL solution of horseradish peroxidase. |
| Unit Definition | That amount of enzyme which catalyses the production of one milligram of purpurogallin in 20 seconds at 20 °C. at a pH of 6.0. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [195373] lot ID: M1035](https://www.mpbio.com/static/version1781469166/frontend/Mpbio/luma/en_US/images/document.png "MP COA [195373] lot ID: M1035")
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