MP Biomedicals α-Tubulin Mouse mAb offers high specificity and potency and consistency against α-Tubulin expressed by human, mouse, and rat species, which has been experimentally validated in Western blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and Immunofluorescence (IF) assays.
Usage Statement
Research use only, not for direct human use.
Key Applications
Western blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
| SKU | 08L100028 |
| Alternate Names | TUBA4A; ALS22; H2-ALPHA; TUBA1 |
| Antibody Type | Monoclonal Antibody |
| Background_information | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta, and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively, spliced transcript variants encoding different isoforms have been found for this gene. |
| Base Catalog Number | L100028 |
| Bioreactivity | This α-Tubulin Mouse mAb offers high specificity and potency and consistency against α-Tubulin expressed by human, mouse, and rat species, which has been experimentally validated in Western blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and Immunofluorescence (IF) assays. |
| Concentration | Please check the vial’s label |
| Conjugate | Unconjugated |
| Formulation Details | In PBS buffer with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3-7.4. This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. This antibody is stable for twelve months from the date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
| Grade | Molecular Grade |
| Host | Mouse |
| Immunogen | Immunogen is a full-length recombinant protein of human α-Tubulin |
| Isotype | IgG |
| Molecular Weight | Observed at 50kDa |
| Pack Size | 100 µL |
| Preparation Method | Affinity purification |
| Species Reactivity | Human, Mouse, Rat |
| Specificity | High specificity against antigens |
| Storage | -20° |
| Typical Working Concentration | WB: 1:5000 - 1:10000; IHC: 1:200 - 1:500; IF: 1:100 - 1:200; IP: 1:100 - 1:200 |
| Unit Definition | P68366 |
| Usage Statement | Research use only, not for direct human use. |