Product Description
MP Biomedicals Rabbit anti-His-tag mAb offers high specificity and potency and consistency against His tag, which has been experimentally validated in Western blotting (WB) and Immunoprecipitation (IP) assays.
Usage Statement
Research use only, not for direct human use.
Key Applications
Western blotting (WB), Immunoprecipitation (IP)
Specifications
| SKU | 08L100033 |
| Alternate Names | His; His tag; His-tag |
| Antibody Type | Monoclonal Antibody |
| Background_information | Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies. |
| Bioreactivity | This Rabbit anti-His-tag mAb offers high specificity and potency and consistency against His tag, which has been experimentally validated in Western blotting (WB) and Immunoprecipitation (IP) assays. |
| Concentration | Please check the vial’s label |
| Conjugate | Unconjugated |
| Formulation Details | In PBS buffer with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3-7.4. This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. This antibody is stable for twelve months from the date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
| Grade | Molecular Grade |
| Host | Rabbit |
| Immunogen | Immunogen is a synthetic peptide corresponding to His tag. |
| Isotype | IgG |
| Molecular Weight | Observed at 16 kDa |
| Pack Size | 100 µL |
| Preparation Method | Affinity purification |
| Solubility | Dissolves in Tris or PBS buffer immediately |
| Specificity | High specificity against antigen tag |
| Storage | -20° |
| Typical Working Concentration | WB: 1:2000 - 1:10000; IP: 1:2000 - 1:10000 |
| Usage Statement | Research use only, not for direct human use. |
Documents
Certificate of Analysis (COA)
Datasheet
Datasheet
English (United States)
Brochures
Brochures
English (United States)
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