Rabbit anti-DDDDK Tag (FLAG Tag) mAb
CA$383.10
In stock
SKU: 08L100031
Product Description
MP Biomedicals DDDDK-Tag (FLAG Tag) Rabbit mAb offers high specificity and potency and consistency against FLAG Tag, which has been experimentally validated in Western blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), and Immunofluorescence (IF) assays.
Usage Statement
Research use only, not for direct human use.
Key Applications
Western blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and Immunofluorescence (IF) assays
Specifications
| SKU | 08L100031 |
| Alternate Names | DDDDK; DDDDK tag; DDDDK-tag |
| Antibody Type | Monoclonal Antibody |
| Background_information | FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography. A FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting. |
| Base Catalog Number | L100031 |
| Bioreactivity | This DDDDK-Tag (FLAG Tag) Rabbit mAb offers high specificity and potency and consistency against FLAG Tag, which has been experimentally validated in Western blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), and Immunofluorescence (IF) assays. |
| Concentration | Please check the vial’s label |
| Conjugate | Unconjugated |
| Formulation Details | In PBS buffer with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3-7.4. This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. This antibody is stable for twelve months from the date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
| Grade | Molecular Grade |
| Host | Rabbit |
| Immunogen | Immunogen is a synthetic peptide corresponding to DDDDK tag. |
| Isotype | IgG |
| Pack Size | 100 µL |
| Preparation Method | Affinity purification |
| Protein Tag | FLAG Tag |
| Solubility | Dissolves in Tris or PBS buffer immediately |
| Storage | -20° |
| Typical Working Concentration | WB: 1:500 - 1:2000; IHC: 1:50 - 1:500; IF: 1:50 - 1:500; IP: 1:50 - 1:500 |
| Usage Statement | Research use only, not for direct human use. |
Documents
Certificate of Analysis (COA)
Datasheet
Datasheet
English (United States)
Brochures
Brochures
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