Recombinant SARS-CoV-2 Papain-like Protease (His tag)
SKU: 08L100014
Recombinant SARS-CoV-2 papain-like protease is produced by E. coli expression system. The target protein is expressed with sequence (Glu1564-Lys1878) of SARS-CoV-2 papain-like protease (Accession #YP_009725299.1) fused with an initial Met, a 6×His tag at the N-terminus.
Usage Statement
Research use only, not for direct human use.
Key Applications
ELISA, SPR, SDS-PAGE, Western Blotting, Immunoprecipitation, Immune Blocking, Binding Assay
| SKU | 08L100014 |
| Alternate Names | PLPro; PL-PRO; pp1a; Papain-like Protease; Replicase polyprotein 1a; ORF1a polyprotein; Plpro |
| Concentration | Please check the vial’s label |
| Conjugate | Unconjugated |
| Endotoxin | < 1.0 EU/μg of the protein by LAL method |
| Formulation Details | Supplied as a 0.22 μm filtered solution in 20mM Tris, 20% Glycerol, 10mM β-Me, pH 7.5. |
| Grade | Molecular Grade |
| Host | E. coli |
| Pack Size | 100 μg |
| Preparation Method | Affinity Chromatography |
| Protein Tag | N-6×His |
| Purity | > 97% by SDS-PAGE |
| Solubility | Reconstitute it with sterile PBS or 10 mM Tris buffer. Addition of a carrier protein (such as 0.05-0.1% BSA) is recommended for long-term storage. |
| Species Reactivity | Human, Mouse, Rat |
| Storage | -20° |
| Usage Statement | Research use only, not for direct human use. |