Concanavalin A, FITC conjugated

Name: Concanavalin A, FITC conjugated
SKU: 02153245-CF
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SKU: 02153245-CF

Key features and details

Concanavalin A, Fitc Conjugated

Synonyms

Con A

CAS Number:

Not applicable

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Product Description

Concanavalin A, Fitc Conjugated

Application Notes

Concanavalin A used to examine tissue sections. It is conjugated to FITC, allowing fluorescent detection in binding assays for example detection of glycoproteins containing α−D-mannose or α−D-glucose. It is also used in blood grouping and erythrocyte polyagglutination studies, mitogenic stimulation of lymphocytes, ymphocyte subpopulation studies, fractionation of cells and other particles, histochemical studies of normal and pathological conditions.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Fluorescent detection in binding assays

Specifications

SKU	02153245-CF
Alternate Names	Con A
Application Notes	Concanavalin A used to examine tissue sections. It is conjugated to FITC, allowing fluorescent detection in binding assays for example detection of glycoproteins containing α−D-mannose or α−D-glucose. It is also used in blood grouping and erythrocyte polyagglutination studies, mitogenic stimulation of lymphocytes, ymphocyte subpopulation studies, fractionation of cells and other particles, histochemical studies of normal and pathological conditions.
Base Catalog Number	153245
Biochemical Physiological Actions	Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
CAS #	Not applicable
Conjugate	FITC conjugate
Format	Frozen Solution
Hazard Statements	H317
Personal Protective Equipment	Dust mask, Eyeshields, Faceshields, Gloves
Preparation Method	Gel filtration performed after conjugation to remove free FITC. Buffer: 0.05 M Tris- 0.15 M NaCl-0.004 M CaCl2, pH 7.0-7.2. contains sodium azide as a preservative.
Safety Symbol	GHS07
Source	Jackbean
Specificity	(Carbohydrate) α-D-Mannose; α-D-Glucose; Branched mannose
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.