Anti-Galactosidase, purified monoclonal antibody [subclass IgG2a(k)] was purified from ascites of a mouse hybridoma and recognizes E. coli β-galactosidase. Recognizes the native and denatured enzyme near the C-terminal end.
Application Notes
Monoclonal anti-β-galactosidase antibodies may be used in dot blot, western blot and immunocytochemistry. This antibody may also be used for immunochemical assays such as ELISA, immunoblot and immunohistochemistry by using the step-wise procedure or by the preparation of β-galactosidase anti-β galactosidase (BBAG) complex. Additionally, this product is suitable for the detection and purification of recombinant fusion proteins encoded by cloning vectors containing the β-galactosidase gene. Monoclonal anti-β-galactosidase antibody is also used for the immunoenzymatic staining of bone marrow and blood smears, and tissue sections.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Dot Blot | Immunoblot | Immunocytochemistry | Immunocytochemistry | Immunoblot | Dot blot
| SKU | 08633651 |
| Antibody Type | Monoclonal Antibody |
| Application Notes | Monoclonal anti-β-galactosidase antibodies may be used in dot blot, western blot and immunocytochemistry. This antibody may also be used for immunochemical assays such as ELISA, immunoblot and immunohistochemistry by using the step-wise procedure or by the preparation of β-galactosidase anti-β galactosidase (BBAG) complex. Additionally, this product is suitable for the detection and purification of recombinant fusion proteins encoded by cloning vectors containing the β-galactosidase gene. Monoclonal anti-β-galactosidase antibody is also used for the immunoenzymatic staining of bone marrow and blood smears, and tissue sections. |
| Base Catalog Number | 633651 |
| Clone Name | 6A-11 |
| Concentration | 2.2 mg/mL |
| Conjugate | Unconjugated |
| Format | lyophilized |
| Formulation Details | Purified |
| Grade | Molecular Grade |
| Host | Mouse |
| Immunogen | Betagalactosidase from E. coli |
| Isotype | IgG2a, kappa |
| Pack Size | 100 µg |
| Purity | Immunoaffinity Chromatography |
| Solubility | Soluble in water |
| Species Reactivity | Bacteria |
| Specificity | The monoclonal antibody recognizes E. coli b-Galactosidase. In a dot blot assay the antibody reacts with both native and denatured b-Galactosidase. |
| Typical Working Concentration | Dilutions:Tissue Staining: 1:5 - 1:20 Immunoblotting: 1:10 - 1:50 Elisa/FIA: 1:40 - 1:200 Dot Blot: 1:5000 Immunoprecipitation: 25 mg/ml |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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