SPINeasy DNA Kit for Feces is a high-performance feces gDNA extraction kit based on spin-column technology. It enables quick isolation of gDNA from feces in less than 30 min. Briefly, feces samples are placed into Lysing Matrix E tubes and used with FastPrep® Instruments from MP Biomedicals to effectively lyse various cells including bacteria, fungi, viruses, protists and more. A series of specially formulated reagents without phenol and chloroform were developed to remove proteins and other contaminants. Column F1 provided in the kit has high binding capacity and selectivity for gDNA. The combined components of the kit provide an indispensable tool for extracting high yields of pure gDNA from different species. Extracted gDNA is free from inhibitors and ready for downstream analyses such as PCR, restriction enzyme digestion and sequencing.
Product Highlight
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Yield : Dedicated lysing beads with FastPrep instrument to effectively rupture cells and thus promise good yield of DNA.
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Purity : Specially formulated inhibitor removal buffers to greatly improves the purity and the extracted DNA can be directly used for downstream applications.
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Speed : Easy to use spin-column method and completes DNA extraction as fast as 25min/run
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Wide Application : Suitable for all types of feces and intestinal content
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Safety : Extraction process does not involve toxic chemicals such as phenol and chloroform
Application Notes
Extract high yield of pure gDNA from different species, including human, mouse, swine, chicken, cow, goat and others. Extracted gDNA is free from inhibitors and ready for downstream analyses such as PCR, restriction enzyme digestion and sequencing.
Usage Statement
Research Use Only (RUO)
Key Applications
Used to isolate gDNA from feces and intestinal content.
| SKU | 116531050 |
| Application Notes | Extract high yield of pure gDNA from different species, including human, mouse, swine, chicken, cow, goat and others. Extracted gDNA is free from inhibitors and ready for downstream analyses such as PCR, restriction enzyme digestion and sequencing. |
| Base Catalog Number | 6531050 |
| Components | 50 x 2 mL Lysing Matrix E, 60 mL Pre-Wash Buffer,60 mL Lysis Buffer F1, 8 mL Lysis Buffer F2, 550 μL RNase A solution, 15 mL Inhibitor Removal FS, 15 mL Binding Buffer FS, 9 mL Wash Buffer F1, 6 mL Wash Buffer F2, 10 mL DES buffer, 50 x Column F1,50 x 2.0 mL Collection Tubes and 50 x 1.5 mL Collection Tubes . |
| Pack Size | 50 preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Storage | Store in a dry environment at room temperature (15-30 °C) |
| Usage Statement | Research Use Only (RUO) |
