Potassium Ferrocyanide Trihydrate
Application Notes
Potassium ferrocyanide is used to determine the concentration of potassium permanganate, a compound often used in titrations based on redox reactions. Potassium ferrocyanide is used in a mixture with potassium ferricyanide and phosphate buffered solution (PBS) to provide a buffer for X-Gal, which is used to cleave Beta-galactosidase, giving a bright blue visualization where an antibody (or other molecule), conjugated to Beta-gal, has bonded to its target.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
SKU | 02152560-CF |
Alternate Names | Yellow prussiate; Potassium hexacyanoferrate(II) trihydrate |
Application Notes | Potassium ferrocyanide is used to determine the concentration of potassium permanganate, a compound often used in titrations based on redox reactions. Potassium ferrocyanide is used in a mixture with potassium ferricyanide and phosphate buffered solution (PBS) to provide a buffer for X-Gal, which is used to cleave Beta-galactosidase, giving a bright blue visualization where an antibody (or other molecule), conjugated to Beta-gal, has bonded to its target. |
Base Catalog Number | 152560 |
Biochemical Physiological Actions | The ferrocyanide species is membrane impermeable and can be used to assess redox activity of membrane-bound structures and mechanisms of electron transport across membranes. This approach was employed to demonstrate that Potassium ferrocyanide performs reducing activation of membrane-bound dopamine β-monooxygenase from the exterior face of adrenal vesicles, providing elucidation of native reduction-activation mechanisms for dopamine β-monooxygenase regulation. |
CAS # | 14459-95-1 |
EC Number | 237-722-2 |
Format | Powder |
Grade | ACS grade |
Hazard Statements | H412 , Contact with acids liberates very toxic gas. |
Insoluble Matter | ≤0.005% |
Molecular Formula | K4Fe(CN)6·3H2O |
Molecular Weight | 422.391 g/mol |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |