Pyronin Y
Application Notes
Pyronin Y is used with methyl green for staining plasma cells and bacteria in mammalian tissue. Also used in 0.05% concentration to stain RNA. Transfer RNA (tRNA) will complex with Pyronin Y at a 1 : 10 molar ratio when analyzed by polyacrylamide gel electrophoresis. tRNA (4 to 8 µg) when complexed with Pyronin Y is visible as a red band during electrophoresis. Pyronin Y can also be used in correlating RNA content by flow cytometry. In addition, Pyronin Y solutions may be utilized in staining liver cells that have been fixed in Carnoy's fluid.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Nucleic Acid Electrophoresis Stains | Staining liver cells
| SKU | 04800506 |
| Alternate Names | Pyronin G; Pyronin Yellow; C.I. 45005; N-(6-(Dimethylamino-3H-Xanthen-3-Ylidene)-N-Methyl-Methanaminium Chloride; Pyronin J; cationic xanthene dye |
| Application Notes | Pyronin Y is used with methyl green for staining plasma cells and bacteria in mammalian tissue. Also used in 0.05% concentration to stain RNA. Transfer RNA (tRNA) will complex with Pyronin Y at a 1 : 10 molar ratio when analyzed by polyacrylamide gel electrophoresis. tRNA (4 to 8 µg) when complexed with Pyronin Y is visible as a red band during electrophoresis. Pyronin Y can also be used in correlating RNA content by flow cytometry. In addition, Pyronin Y solutions may be utilized in staining liver cells that have been fixed in Carnoy's fluid. |
| Base Catalog Number | 800506 |
| Beilstein Registry Number | 3795789 |
| CAS # | 92-32-0 |
| Color Index | 45005 |
| EC Number | 202-147-8 |
| Format | Powder |
| Grade | Molecular Biology Grade |
| Molecular Formula | C17H19N2OCl |
| Molecular Weight | 302.802 g/mol |
| Pack Size | 1 g |
| Personal Protective Equipment | Eyeshields, Gloves, Respirator filter |
| RTECS Number | BQ1450000 |
| Typical Working Concentration | 0.05% (to stain RNA) |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | λ max (50% ethanol): 548 nm(Lit.) |
![MP COA [800506] lot ID: M4621](https://www.mpbio.com/static/version1747301781/frontend/Mpbio/default/en_US/images/document.png "MP COA [800506] lot ID: M4621")