P-Nitrophenyl Phosphate Di(Tris) Salt
Application Notes
p-Nitrophenylphosphate Di(tris) Salt(pNPP) is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 M NaOH and read at 405 nm.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Substrate for assay of alkaline phosphatase
SKU | 02151766-CF |
Alternate Names | 4-Nitrophenyl phosphate di(tris) salt; 4-Nitrophenyl phosphate bis(tris) salt; pNPP |
Application Notes | p-Nitrophenylphosphate Di(tris) Salt(pNPP) is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 M NaOH and read at 405 nm. |
Base Catalog Number | 151766 |
Beilstein Registry Number | 1578142 |
CAS # | 68189-42-4 |
EC Number | 269-198-6 |
Format | Powder |
Molecular Formula | C14H28N3O12P |
Molecular Weight | 461.361 g/mol |
Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
UV Visible Absorbance | λ max (water) 219-223, 307-311 nm |