HIV-1/2 ELISA 4.0

HIV-1/2 ELISA 4.0

Packaging size: 480 Tests

SKU 0723031480
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Product Description

The MP Diagnostics (MPD) HIV-1/2 ELISA 4.0 is an enzyme-linked immunosorbent assay (ELISA) intended for the detection of antibodies to Human Immunodeficiency Viruses (HIV) type 1 (group M - O) or type 2 in human serum or plasma samples. The assay can be utilized for screening of blood donors and/or as an aid in the diagnosis of clinical conditions related to infection with HIV-1 and/or HIV-2 - the etiological agents of the acquired immunodeficiency syndrome (AIDS).

Usage Statement

For Sale Outside the US Only.

SKU 0723031480
Analyte / Target HIV 1&2 antibodies
Base Catalog Number 23031480
Diagnostic Platforms EIA/ELISA
Diagnostic Solutions Infectious Disease
Disease Screened Human Immunodeficiency Virus (HIV)
Evaluation Qualitative
Ig Detected IgA|IgM|IgG
Kit or Assay Type ELISA Kits
Pack Size 480 Tests
Pathogen Human Immunodeficiency Virus (HIV) Types 1 and 2
Pathogen Type Retrovirus
Product Overview The MP Diagnostics HIV-1/2 ELISA 4.0 is an antigen sandwich immunoassay. The wells of the polystyrene microplate strips are coated with recombinant HIV antigens (gp41, gp120, and gp-36) expressed in E.coli. The conjugate is based on a second set of recombinant HIV antigens expressing the same epitopes as the pre-coated antigens, which is conjugated to horseradish peroxidase. Human serum or plasma, are incubated in these coated wells. HIV-1/2 specific antibodies, if present, will bind to the antigens immobilised on the solid phase. After incubation, the wells are thoroughly washed to remove unbound materials and conjugate is added to the wells. The antigens of the conjugate will bind to any antigen-antibody complexes previously formed and excess unbound conjugate are removed by washing. Colourless solutions containing urea peroxide and tetramethylbenzidine are then added to each well. The presence of specific antibodies is indicated by the presence of a blue colour after incubation, which changes to yellow when the colour reaction is terminated by the addition of sulphuric acid. The intensity of the resulting yellow product is measured at 450nm using a spectrophotometer and is proportional to the amount of antibodies present in the specimen.
Regulatory Compliance CE|Ex-US
Sample Type Serum|Plasma
Sample Volume 100 uL
Standards Reactive Control| Non-reactive control
Storage and Handling Store at 2 - 8 degrees Celsius
Usage Statement For Sale Outside the US Only.
Species Reactivity Human