50ML BIGPREP LYSING MATRIX S
Lysing Matrix S, 50 mL tubes contains 150 stainless steel beads. Will not bind nucleic acids.
Metal Bead Lysing Matrix is used to break hard/brittle samples. Specialty applications include RNA and protein extraction from difficult samples where lysis by impaction only is preferable with minimal amounts of mechanical shearing.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Nucleic Acid Isolation |Nucleic Acid Extraction |Cell culture |Proteomics |Purification and Detection |Protein Purification |DNA Purification |RNA Purification |Plant Tissue Processing |Tissue Analysis |Soil Analysis |Fungal Tissue Analysis |Microbiome Studies |Metabolite Extraction |Tissue Homogenization |Isolate Intact Organelles |RNA Extraction from Difficult Samples |Isolation of Intact Bacteria and Viruses from Infected Tissue
|FastPrep Instruments Models||BigPrep (2x50 mL) |CoolBigPrep (2x50 mL) |BigFlex (8x50 mL) |ConeFlex Legacy (allows all FP24 adapters on FP96) |All Metal BigPrep (2x50 mL)|
|Application Notes||Metal Bead Lysing Matrix is used to break hard/brittle samples. Specialty applications include RNA and protein extraction from difficult samples where lysis by impaction only is preferable with minimal amounts of mechanical shearing.|
|Average Size||0.125", 3.175 mm|
|Certifications||DNase and RNase Free|
|Hardness||ROCKWELL C 55 - 64|
|Applications||Nucleic Acid Isolation |Nucleic Acid Extraction |Cell culture |Proteomics |Purification and Detection |Protein Purification |DNA Purification |RNA Purification |Plant Tissue Processing |Tissue Analysis |Soil Analysis |Fungal Tissue Analysis |Microbiome Studies |Metabolite Extraction |Tissue Homogenization |Isolate Intact Organelles |RNA Extraction from Difficult Samples |Isolation of Intact Bacteria and Viruses from Infected Tissue|
|Kit Compatibility||DNA Kits |RNA Kits |Protein Purification Kits |Phoen Gigaprep Kit |MiniPrep Express Matrix |RNA Kit |all gigaprep format kits |Bead Beating Kit |Any Kit Requiring Processing of Biological Samples|
|Material of Construction||Matrix S contains 3.175 mm Stainless Steel, (AISI 440-C) Beads |Tube and Cap, Polypropylene|
|Materials Required Not Supplied||FastPrep ® Instrument |Other High-speed Homogerizer |Vortexer |Centrifuge |Pipetors |Purification Reagents|
|Molecule Enriched||DNA |RNA |Proteins |Metabolites |Other small molecules|
|Personal Protective Equipment||Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|Physical Appearance||50 mL Conical Screw Cap Tube with grinding particles installed|
|Product Families Description||Lysing Matrix is a critical component of any high-speed, reciprocating bead beating sample preparation operation. MP Biomedicals is the innovator in bead beating technology and developer of the FastPrep ® Sample Preparation Systems. We manufacture the widest variety of Lysing Matrix products, optimized by sample type. Sample types include but are not limited to: human and animal and plant tissues; microorganisms like bacterial, yeast and fungi; plant, soil, fecal, plus insects and worms. MP Biomedicals receives matrix particles from proven vendors and ensures the unprocessed material meets high qualitative standards. Matrix particles are then prepared and dispensed under a rigorous set of proprietary conditions that offer customers a premium product that can be immediately used with confidence. Matrices are available separately for use with your own unique buffers, and are also available as components of the complete purification kits. Lysing Matrix is available in ready-to-use format in a variety of grinding vessels including tube sizes of 2 mL, 4.5 mL, 15 mL and 50 mL. High-throughput products include 96-Well Plates and 250 mL grinding bottles. All lysing matrix vessels are standard sizes to fit any homogenizer, centrifuge or vortexer on the market. Lysing Matrix products are available in bulk format for custom filling to your own specifications. For optimal performance and results we recommend using in conjunction with our FastPrep-24 5G Homogenizer and FastPrep ® Extraction Kits. The FastPrep ® System will easily grind, lyse and homogenize any sample type, from routine to the most resistant, in 40 seconds or less. Rapid, quantitative sample disruption results in the highest quality isolation of high MW DNA, stable RNA, biologically active proteins and other small molecules. |FastPrep ® Lysing Matrix tubes are very versatile, consisting of 18 unique matrix compositions to successfully process any sample type! Whatever your sample, we have the solution. Our matrices contain properties that contribute to optimum success in your protocols. Various properties of a lysing matrix particle, whether inherent or selected, can yield a myriad of end-products depending on the dynamic qualities instilled in that particle by said properties. The inherent properties include the hardness, composition, and density, while the selected properties include the shape and the size. Here is a brief description of our properties: Size: The smaller the particles used in the grinding media, the smaller the average particle size and the smaller the lowest-limiting particle size produced during pulverization. (Note: this doesnt mean that the smallest particles produced will be the same size as the smallest matrix particles used during lysis. The particles of the end product will always be much smaller that the smallest matrix particle. Its rather like a bullet penetrating a glass window, producing pieces of shattered glass that are much smaller than the incident bullet.) Thus, matrix particle size should be selected based upon the size of the particles you wish to obtain in your lysate. For example, if your goal is to obtain intact and functional organelles, its best to utilize a larger grinding media. Utilizing a smaller size matrix would be more conducive to lysing smaller structures, such as viral particles and endospores. Shape: The shape of the grinding media is a major determining factor in the modality by which the cells are broken apart. Dull lysis media, such as those with spherical shapes, will complete a majority of the overall lysis by forces of cascade impaction, while the sharper and more jaggedly shaped lysis media will complete a majority of the overall lysis by shearing forces. Shearing forces are good for cracking open difficult-to-lyse samples, or samples that necessitate the isolation of a stable molecule such as DNA. RNA and certain easily denatured proteins are often vulnerable to shearing forces, while other molecules such as DNA, stable proteins (antibodies or collagen for instance), structural polysaccharides, and small molecules (such as small secondary metabolites) are immune to shearing forces, and thus it is practical to use shearing forces when isolating these, so as to eliminate those structures and functions that are vulnerable to such shearing forces. Hardness, Density and Composition: The composition determines two very important qualities: hardness and density, both of which are inherent properties derived from the molecular composition of the matrix particle. The hardness must be greater than that of the sample being pulverized. This is the case for all MP Biomedicals lysing matrices, so this variable is not as significant. The density is more important. The denser the matrix particles, the more effective they are at grinding up spores or tough tissues with dense extracellular matrices. Its also important that the matrix particles being used are denser than the solution in which they are homogenizing, lest they simple float on top and fail to function in lysis or homogenization. Other qualities also accompany the matrix due to molecular structure. Some examples include electrical conductivity, melting point, and cross-reactivity, but these and other unmentioned inherent molecular properties are essentially insignificant due to our careful selection of the compositions of our lysing matrix particles. A Word About Knoop and Mohs: Materials chosen for use in FastPrep ® Lysing Matrix products are carefully selected based on several criteria (discussed above). A key parameter is the hardness of the material, which can be evaluated by standard assays, which are reported for each of our quality reagents. The Knoop hardness test is a test for mechanical hardness of brittle materials or thin sheets. A small indentation is made in the surface using a diamond point applied with known force/time. The resulting indentation is measured using a microscope and scored with values typically from 100 to 1000, with increasing values being indicative of increase of material hardness. The Mohs scale defines the scratch resistance of various minerals, assessed as the ability of a harder material to scratch a softer material. Values typically range between 1 and 10, with diamond (for example having a Mohs scale score of 10).|
|Product Overview||Each impact-resistant 50 mL mL tube contains one hundred fifty (150) 1/8" (3.175 mm) diameter stainless steel beads. Use to break hard/brittle samples. Specialty applications include RNA and protein extraction from difficult samples where lysis by impaction only is preferable with minimal amounts of mechanical shearing.|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation |Soil Science |Proteomics |Metagenomics |Metabolomics |Food Sciences |Forensics |Plant Pathology |Communicable Disease |Cancer |Agricultural |Microbiomics |Epigenetics|
|Sample Type||All Samples |Tough Tissues |Skeletal Muscle |Lung |Heart |Bone |Seeds |Spores |Plants |Fixed Samples |Food |Roots|
|Sample Volume||up to 7 g per prep|
|Storage and Handling||Store at Room Temperature (15-30 Deg C).|
|Tube Compatibility||50 mL|